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Cornell University

Instructions for excising your gel band

Submit samples
  1. Take an image of the gel before you excise it, email the image to Elizabeth Anderson (eta23 at cornell.edu) indicating the band(s) you have excised and want analyzed. It is very helpful if you can give us an estimation of how much protein is in the band(s) of interest or how much protein you loaded into the well.
  2. To minimize keratin contamination, please wear gloves, and do not touch the gel with your bare hands.
  3. Excise your gel band(s) on a clean glass plate with a clean razor blade. (We clean our instruments with 70% methanol). Conservatively cut at the top and bottom of band of interest to avoid including proteins migrating before/after in your gel band that you are not interested in.
  4. Put the excised gel band into a clean, labeled Eppendorf tube. You can add a small amount of ddH20 or gel fixing solution to help keep the gel piece from drying out. Store at -20C.
  5. Complete an online sample submission form using the "submit samples" button above.
  6. Drop sample(s) off at the Biotechnology building, Lab 141 or mail on ice following our shipping instructions.