We prepare Illumina libraries according to standard protocols that yield nearly 100% success rates. All steps are handled manually. In addition, we will repeat library prep for samples that fail on the first attempt.

DNA sequencing applications

Library preparation methods for DNA samples include:

• Nextera (tagmentation) — can be used for sequencing large and complex genomes, microbiome, and metagenomic samples.
• TruSeq (ligation) – may give more even coverage than Nextera. Standard protocol uses PCR to incorporate UDIs (unique dual indices), with up to 192 UDIs available. PCR-free protocol is also available, with limited UDIs and requiring dPCR for library quantification prior to pooling and sequencing (separate charge).

We can prepare Illumina libraries from a variety of DNA sample types (genomic DNA, pre-enriched DNA such as ChIP, amplified double-stranded cDNA, and more). Please contact us to inquire about services for non-standard sample types. 

Per-sample pricing includes library preparation, quantification, and size QC. Sequencing is required for all DNA libraries.

RNA sequencing applications

The TREx facility offers full-service RNAseq and small RNA sequencing.

High throughput methods

We also offer services for high throughput Illumina sequencing applications (DNA and 3’RNAseq).