Small RNA Sequencing Standard Package
- Sample submission: 100ng - 1ug purified RNA (lower input is sufficient for cell-free RNA; see below).
‘Bioanalyzer QC’ is highly recommended; contact us for information about RNA QC options. - Library preparation: NEBNext Small RNA.
- Sequencing: minimum 10M raw reads.
Deeper sequencing is available on request (extra cost). - Standard analysis: Read preprocessing, quantification of annotated microRNAs (miRdeep2 using miRBase annotations), data QC
reports including total microRNA counts and sample clustering analyses, differential microRNA detection analysis (DESeq2).
Optional: reads can be mapped to a reference genome on request.
Service pricing
| Description | Internal price (Cornell and Cornell affiliates) | External Price |
|---|---|---|
| Samples 1-24 (ea.) | $280 | $460 |
| Samples 25+ (ea.) | $230 | $375 |
| Add 10M Additional small RNA seq Reads | $125 | $205 |
| Description | Internal price (Cornell and Cornell affiliates) | |
|---|---|---|
| Tissue/cell homogenization (per sample) | $15 | Inquire (limited availability) |
| RNA extraction (per sample) | $30 | Inquire (limited availability) |
| RNA sample cleanup (per sample) | $15 | $25 |
| RNA size QC (FA5200) | $22 | $36 |
| Custom lab work (per hour) | $55/hr | $90/hr |
| Consulting and custom analysis (per hour) | $125/hr | $205/hr |
| Custom projects are available on a limited basis and require pre-approval | ||