Quantitative (comparative) proteomics
We offer both labeled and label-free services:
| Categories | Methods | Multiplex | Features | Main limitation |
|---|---|---|---|---|
|
Labeled |
SILAC |
3 |
Most accurate, MS-based quantitation |
Requiring cell culture for labeling |
|
Dimethyl |
3 |
MS-based quantitation suitable for limited materials; flexibility |
Limit for 3-plex |
|
|
iTRAQ |
up to 8 |
Excellent MS/MS-based quantitation; flexibility High quantitation accuracy and in-depth coverage using the latest RTS SPS MS3 technology |
Sample preparation is performed in individual samples for labeling and then the labeled samples are pooled. This could introduce technical sample-to-sample variation. |
|
|
TMT |
up to 18 |
Excellent MS/MS-based quantitation; flexibility, high throughout High quantitation accuracy and in-depth coverage using the latest RTS SPS MS3 technology |
Sample preparation is performed in individual samples for labeling and then the labeled samples are pooled. This could introduce technical sample-to-sample variation. |
|
|
Label-free |
XIC |
1 |
MS-based precursor ion intensities or peak areas |
Large quantitation variation, requiring optimized LCs |
|
Spectral counting or emPAI |
1 |
MS/MS-based spectra counts or pep ID numbers |
Large quantitation variation, requiring optimized LCs |
|
|
SRM |
1 |
Super-accurate MS/MS quantitation |
Requiring synthesis of standard peptides and known targeted peptides |
Our full service includes sample preparation, sample analysis and data interpretation. If you want to do the sample preparation or data interpretation yourself, let us know during the project consultation.
In addition, we provide equipment and software support for 2D gel-based quantitation analysis (2DE or 2D DIGE), upon requested for some specific projects.
The service fees are broken down in the tables below.
Service pricing
| Description | Internal price (Cornell and Cornell affiliates) | External price |
|---|---|---|
| Bradford Assay (set up) | $40 | $65.60 |
| Bradford Assay (per sample) | $6 | $9.84 |
| Affinity depletion of serum high abundant proteins by column (per sample) | $130 | $213.20 |
| Affinity depletion of serum high abundant proteins by ProteoMiner (per sample) | $70 | $114.80 |
| Peroteominer Supplies (per sample) | $90 | $147.60 |
| iTRAQ 4-plex reagent (per set of samples) | $300 | $492 |
| iTRAQ 4-plex labeling and sample preparation (per set of samples) | $550 | $902 |
| iTRAQ 8-plex reagent (per set of samples) | $600 | $984 |
| iTRAQ 8-plex labeling and sample preparation (per set of samples) | $780 | $1,279.20 |
| TMT 6-plex reagent (per set of samples) | $380 | $623.20 |
| TMT 6-plex labeling and sample preparation (per set of samples) | $650 | $1,066 |
| TMT10-plex reagent (per set of samples) | $750 | $1,230 |
| TMT 10-plex labeling and sample preparation (per set of samples) | $860 | $1,410.40 |
| TMT 11-plex reagent (per set of samples) | $880 | $1,443.20 |
| TMT 11-plex labeling and sample preparation (per set of samples) | $950 | $1,558 |
| TMT 16-plex reagent (per set of samples) | $1,100 | $1,804 |
| TMT 16-plex labeling and sample preparation (per set of samples) | $1,600 | $2,132 |
| TMT 18-plex reagent (per set of samples) | $1,300 | $2,132 |
| TMT 18-plex labeling and sample preparation (per set of samples) | $1,500 | $2,460 |
| Buffer exchange by ultrafiltration/dialysis/spin column (per sample) | $25 | $41 |
| Spin filtration (per sample) | $15 | $24.60 |
| Gel-based protein quantitation for shotgun 10-15 well (per set of samples) | $200 | $328 |
| Gel-based protein quantitation for shotgun 26 well (per set of samples) | $300 | $492 |
| dimethylation duplex labeling (per set of samples) | $350 | $574 |
| dimethylation triplex labeling (per set of samples) | $500 | $820 |
| mini SDS-PAGE (precast, 10-15 well) running, stain and imaging (per set of samples) | $150 | $246 |
| mini SDS-PAGE (precast, 26 well) running, stain and imaging (per set of samples) | $235 | $385.40 |
| Gel Imaging on ChemiDoc (per hour) | $65 | $106.6 |
| Gel Quant analysis for 1D gel bands by Image Lab 6.1 (per gel) | $65 | $106.60 |
| gel band excise (per band manual) | $5 | $8.20 |
| In-solution digestion (set up) | $100 | $164 |
| In-solution digestion (per sample) | $55 | $90.20 |
| Strap-Based (micro) in-solution digestion (100ug) per sample | $75 | $123 |
| Strap-Based (mini) in-solution digestion (100ug-300ug) per sample | $90 | $147.60 |
| Strap-Based (midi) in-solution digestion (>300ug) per sample | $110 | $180.40 |
| MCX/MAX Cartridge (manual) (per sample) | $70 | $114.80 |
| HRP Cartridge manual fractionation (per sample) | $100 | $164 |
| Sample Prep Optimization (non SOP) per hour | $70 | $114.8 |
| Description | Internal price (Cornell and Cornell affiliates) | External price |
|---|---|---|
| high pH reverse phase with fractions collection (per sample) | $275 | $451 |
| nanoLC-MS/MS: 90-120min gradient in Orbitrap Fusion/Eclipse (per sample) | $250 | $410 |
| nanoLC-MS/MS: 90-120min gradient in Orbitrap Eclipse RTS MS3 (per sample) | $275 | $451 |
| MRM-LC-MS/MS for peptide quantitation 30min gradient (per sample) | $120 | $196.8 |
| MRM-LC-MS/MS for peptide quantitation->=60min gradient (per sample) | $200 | $328 |
| Repeat LC/MS/MS for protein quan/ID | $200 | $328 |
| Description | Internal price (Cornell and Cornell affiliates) | External price |
|---|---|---|
| Quantitation analysis for label-free nanoLC-MS/MS (per hour) | $150 | $246 |
| Customized database interrogation (per database) | $80 | $131.20 |
| Additional database search and report (per hour) | $80 | $131.20 |
| Database search training (per hour) | $165 | $270.60 |
| Database search and report for externally generated raw data files (per search) | $225 | $369 |
| Raw & result files uploading to public database (PRIDE, Metabolights) | $450 | $738 |