Protein identification
Our full service includes sample preparation, sample analysis and data interpretation. If you want to do the sample preparation or data interpretation yourself, let us know during the project consultation.
The service fees are broken down in the tables below.
To provide high quality protein ID results, we recommend the following:
For gel separated samples:
-
regular or colloidal Coomassie Blue, or Sypro ruby-based stains (preferred method)
- mass spectrometry compatible silver-stain (acceptable but not recommended for subsequent protein IDs)
For solution-based samples:
-
a SDS-gel image, to allow us to assess the protein complexity, dynamic range, and total amount of proteins in the samples.
Service pricing
| Description | Internal price (Cornell and Cornell affiliates) | External price |
|---|---|---|
| Spin filtration (per sample) | $15 | $24.60 |
| Spin cartridge desalt | $25 | $41 |
| mini SDS-PAGE (precast, 10-15 well) - running, stain and imaging per gel | $150 | $246 |
| mini SDS-PAGE (precast, 26 well) - running, stain and imaging per gel | $235 | $385.40 |
| 1D mini gel CCB stain, excluding reagent cost for fluorescence stain (per gel) | $25 | $41 |
| Gel imaging on Chemidoc(per hour) | $65 | $106.60 |
| Gel Quant analysis for 1D gel bands by Image Lab 6.1 (per gel) | $65 | $106.60 |
| gel band excise (per band manual) | $5 | $8.20 |
| In-gel Digest/Extraction (set up) | $100 | $164 |
| In-gel Digest/Extraction plus filtration (per sample) | $70 | $114.80 |
| In-solution digestion (set up) | $100 | $164 |
| In-solution digestion (per sample) | $55 | $90.20 |
| second enzyme digestion (per sample) | $25 | $41 |
| ZipTip, manual (per sample) | $15 | $24.60 |
| SPE Cleanup, manual (per sample) | $27 | $44.28 |
| MCX/MAX Cartridge, manual (per sample) | $70 | $114.80 |
| Description | Internal price (Cornell and Cornell affiliates) | External price |
|---|---|---|
| nanoLC-MS/MS: 30min gradient in Orbitrap Fusion/Eclipse (per sample) | $150 | $246 |
| nanoLC-MS/MS: 60min gradient in Orbitrap Fusion/Eclipse (per sample) | $200 | $328 |
| nanoLC-MS/MS: 90-120min gradient in Orbitrap Fusion (per sample) | $250 | $410 |
| nanoLC-MS/MS: 240min gradient in Orbitrap Fusion/Eclipse (per sample) | $400 | $656 |
| Infusion ESI-MS/MS for protein IDs and PTMs (per sample) | $130.00 | $213.20 |
| microLC-MS/MS (15min gradient) for rapid protein IDs (per sample, 1-10 samples) | $90 | $147.60 |
| microLC-MS/MS (15min gradient) for rapid protein IDs (per sample, >10 samples) | $75 | $123 |
| Repeat LC/MS/MS for protein quan/ID | $200 | $328 |
| Target-based LC-MS/MS run for protein IDs or quan or PTMs | $300 | $492 |
| Description | Internal price (Cornell and Cornell affiliates) | External price |
|---|---|---|
| Customized database interrogation (per database) | $80 | $131.20 |
| Additional database search (per hour) | $80 | $131.2 |
| Database search training (per hour) | $165 | $270.60 |
| Database search and report for externally generated raw data files (per search) | $225 | $369 |
| Raw & result files uploading to public database (PRIDE, Metabolights) | $450 | $738 |