DNA sequencing (Sanger)
First option: Full service
You bring us your plasmid or your PCR product. We perform the Big Dye Terminator Cycle Sequencing, the Big Dye Terminator cleanup, and the template sequencing.
Some benefits of the full service:
- For difficult-to-sequence templates or samples needing special attention, we can modify our standard conditions*.
- For templates that have a high GC-content or large constructs like BACs, we use customized sequencing chemistries to generate the best results.
*Additional charges may apply.
Instructions to prepare samples for Full Service DNA sequencing
Second option: Ready-to-Load
You perform the Big Dye reaction and clean-up*. You bring us the samples ready to sequence in a plate that we load into the sequencer.
*Optionally, we can perform the cleanup for an additional fee.
Instructions to submit your Ready-to-Load samples
For all Sanger options: If the template you provide is high quality and in sufficient quantity, we routinely produce up to 900 bases per reaction.
Service pricing
| Description | Internal price (Cornell and Cornell affiliates) | External price |
|---|---|---|
| Full Service Sequencing, plasmid or PCR product, per sample, template premixed with sequencing primer | $4.30 | $7.10 |
| Full Service Sequencing, plasmid or PCR product, per 96-well plate, templates premixed with sequencing primer, 94 samples | $315.00 | $515.00 |
| Special Conditions--dGTP addition, BAC sequencing, added to the single sample sequencing cost, per reaction | $6.00 | $10.00 |
| Universal Primer Addition for Full Service Sequencing | $1.50 | $2.50 |
| Tube Transfer for Full Service Sequencing | $1.00 | $1.60 |
| Ready-to-Load Sequencing, per 96-well plate | $50.00 | $80.00 |
| Column Cleanup for Ready-to-Load Sequencing, per 96-well plate | $80.00 | $130.00 |