DNA/RNA Extraction
DNA and RNA extraction in plate format
Designed for large projects, this service is only available if you intend to follow up with our high-throughput library preparation services. We charged a fixed price for up to 95 samples in 96-well plate format, including at least 1 blank well.
- DNA extraction: average DNA yield of 2-7 µg per sample.
- RNA extraction: average total RNA yield of 2-10 µg per sample.
Our protocols are optimized for DNA/RNA extraction from plant tissue. DNA extraction is limited to plant leaf tissue only. Occasionally, we perform RNA extraction from non-plant tissues; please contact us to inquire. Quantification of DNA/RNA concentration is invoiced separately.
RNA extraction for full-service RNAseq
Please see our page for TREx services; support for RNA extraction is limited to labs that do not have the appropriate facilities for RNA extraction.
Sample clean-up
Samples that show evidence of impurities may require clean-up prior to initiating Illumina library prep. If you submit DNA or RNA that does not meet sample purity requirements, we will recommend sample clean-up using commercial columns or magnetic beads. Quantification of DNA/RNA concentration is invoiced separately.
Sample concentration or dilution
Samples that do not meet our requirements for input concentration may require initial concentration or dilution. This service may be added for our high-throughput library preparation services to normalize input for a large batch of samples as needed.
Service pricing
Description | Internal price (Cornell and Cornell affiliates) | External price |
---|---|---|
DNA extraction: plant leaf tissue only (96 well plate)** | $650 | $1065 |
RNA extraction: limited tissues & species* (96 well plate)** | $650 | $1065 |
DNA cleanup (96 well plate) | $275 | $450 |
DNA cleanup (per sample) | $25 | $40 |
Nucleic acid quantification (96-well plate) | $135 | $220 |
Nucleic acid quantification (per sample) | $5 | $8 |
DNA/RNA sample dilution (96-well plate) | $55 | $90 |
Custom lab work (per hour) | $55 | $90 |
* Pilot study recommended for species or tissues we have not worked with previously. ** Plate must include at least one blank negative control (empty well). |